An immunoassay system to investigate epidemiology of Rocahepevirus ratti (rat hepatitis E virus) infection in humans
نویسندگان
چکیده
Background & AimsRat hepatitis E virus (Rocahepevirus ratti; HEV-C1) is an emerging cause of that divergent from conventional human-infecting HEV variants (Paslahepevirus balayani; HEV-A). Validated serological assays for HEV-C1 are lacking. We aimed to develop a parallel enzymatic immunoassay (EIA) system identifies individuals with exposure. also conduct the first seroprevalence study in humans using this validated EIA system.MethodsExpressed HEV-A (HEV-A4 p239) and (HEV-C1 p241) peptides were characterized. Blood samples simultaneously tested HEV-A4 p239 p241 IgG EIAs. An optical density (OD) cutoff-based interpretation algorithm identifying seropositive or was RT-PCR-positive infection sera. This used measure 599 solid organ transplant (SOT) recipients age-matched immunocompetent individuals.ResultsBoth formed virus-like particles. When run EIAs, distinct clusters minimal overlap 2D plot OD values. The final showed high agreement RT-PCR results (Cohen’s κ = 0.959) able differentiate sera accuracy 94.2% (95% CI: 85.8 – 98.4%). 7/599 (1.2%) among SOT 4/599 (0.7%) individuals. 5/11 (45.5%) these patients had history transient unknown cause.ConclusionsHEV-C1 exposure identified 11/1198 (0.92%) Hong Kong indicating endemic estimate humans. valuable tool investigating epidemiology risk factors infection.Lay summaryRat has recently been discovered infect humans, but antibody tests lacking making it difficult gauge how common is. developed test can identify past rat rates found approximately 1% all people exposed previously.
منابع مشابه
Sero-Epidemiology of Hepatitis E Infections in patients with chronic Hepatitis C virus infection in Jahrom, Southern Iran
Background and Aims: Co-infection of hepatitis E virus (HEV) in chronic hepatitis C virus (HCV) infected patients can develop and increase liver disease and hepatic complications in the world. The purpose of this study was to assess the prevalence of HEV infection in patients suffering from chronic HCV infection. Materials and Methods: Cross-sectional study testing anti-HEV antibodies in serum...
متن کاملEpidemiology of mammalian hepatitis E virus infection.
Mammalian hepatitis E virus (HEV), the etiological agent of hepatitis E in humans, is a recently discovered infectious agent. It was identified for the first time in 1983 using electron microscopy on a faecal specimen of a person infected with non-A, non-B enterically-transmitted hepatitis. Based on retrospective and prospective studies, HEV was long described as one of the leading causes of ac...
متن کاملEpidemiology of hepatitis A and E virus infection in Brazil.
This review has the objective to discuss the epidemiological aspects of the enterically transmitted hepatitis A and E in Brazil. The prevalence of hepatitis A varies greatly in different Brazilian regions, from 56% in South and Southeast to 93% in North region (Manaus, Amazon). Such differences are also found in different socioeconomic levels among age groups. A significantly higher prevalence ...
متن کاملSeroprevalence of Hepatitis E Virus Infection among Patients with Acute Hepatitis Symptoms in Ahvaz, Iran
Background and Aims: Hepatitis E virus (HEV) is considered as one of the common causes of acute viral hepatitis in the world, particularly in the developing countries. It also causes fulminant hepatitis in pregnant women. Although HEV infection in Iran is endemic, there are numerous reports of epidemics caused by this infection in this part of the world. This study aimed to determine seropreval...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: JHEP reports
سال: 2023
ISSN: ['2589-5559']
DOI: https://doi.org/10.1016/j.jhepr.2023.100793